Core unit model development

Leader:
Dr. Andrea Rossi tl_files/bilder/mail.gif
Phone: +49 (0)211-3389-379

Scientist:

Dr. Björn Hiller tl_files/bilder/mail.gif

Technical Assistance:

Marie Brauers, BTA tl_files/bilder/mail.gif
Olivia van Ray, BTA tl_files/bilder/mail.gif

Research profile

The main task of the core unit is to further develop existing and to establish new organotypic human cultures, to use cutting-edge techniques to manipulate the genome of these models as well as the generation of genetically modified animal models (e.g. C. elegans). In the medium term, the development of the following models is planned for research field 1: lung models based on primary cells which allow the exposure with airborne particles and nanoparticles in the air flow; for research field 3: iPS cell based human, three dimensional brain organoids; for research field 2 and 4: long-term cultivable (up to 6 months), human, three dimensional skin models from fibroblasts and keratinocytes.

Selected publications

El-Brolosy M, Rossi A, Kontarakis Z, Kuenne C, Günther S, Fukuda N, Khrievono K, Boezio G, Takacs C, Lai SL, Fukuda R, Gerri C, Giraldez J, Didier YR, Stainier DYR: Genetic compensation triggered by mutant mRNA degradation. Nature 2019. (Highlighted by Jan Philip Junker Detouring the roadblocks in gene expression) [pubmed]

Rossi A, Gauvrit S, Stainier DY: Regulation of Vegf signaling by natural and synthetic ligands. Blood 128(19): 2359-2366, 2016. [pubmed] (Highlighted by Tatiana Byzova “Fishing” out the real VEGFs)

Rossi A, Kontarakis Z, Gerri G, Nolte H, Hölper S, Krüger M, Stainier DY: Genetic compensation induced by deleterious mutations but not gene knockdowns. Nature 524(7564): 230-233, 2015. [pubmed]

Stainier DY, Kontarakis Z, Rossi A: Making sense of anti-sense data. Dev Cell 32(1): 7-8, 2015. [pubmed]

Rossi A, Moritz T, Ratelade J, Verkman AS: Super-resolution imaging of aquaporin-4 orthogonal array of particles in cell membranes. J Cell Sci 125(18): 4405-4412, 2012. [pubmed]